Analysis of local expression of inflammatory cytokines genes in patients with gastroesophageal reflux disease and its association with types and number of gastroesophageal refluxes

The aim of the study was to evaluate gene expression proinflammatory cytokines in esophageal mucosa patients with erosive esophagitis (EE) and non-erosive form (NERD) gastroesophageal reflux disease (GERD) perform correlation analysis between genes types number refluxes. Methods. This a single-center prospective study. Esophageal samples were taken from lower part esophagus during endoscopy. Expression IL-1b, IL-10, IL-18, TNFA, TLR4, GATA3, CD68 beta-2 macroglobulin assessed ImmunoQuantex assays. Multichannel intraluminal pH-impedance studies performed Ohmega recorder 2pH-6impedance catheters. Results. Data 60 (females - 34; mean age (M±SD) 54.6 years, 20 esophagitis) available for analysis. In those EE, there higher IL-18 (5.89±0.4 vs 5.28±1.1, p=0.04), GATA3 (2.92±0.86 2.23±0.96, p=0.03) genes, inflammatory index (92.12±32.41% 70.1±30.8%, p=0.02) compared NERD. Direct found acid exposure time IL-1b (Spearman rank R = 0.29), (R=0.31), TNFA (R=0.35), (R=0.34), TLR4 (R=0.29), (R=0.37) (R=0.3). Indirect revealed рН expressions (R= -0.28), -0.33), -0.39) -0.17). No genes’ Conclusions: cytokines’ differ EE There is studied acidity.